Finnigan MAT 252 IRMS
With the Finnigan MAT 252 you can perform gas isotope ratio measurements of H/D, 13C/12C, 15N/14N, 18O/16O, 32S/33S/34S.
The basic instrument is equipped with a dual inlet system for classical applications. It is of a modular design for the adaptation of different inlet modules thus enabling a configuration of the instrument tailored to the requirement of the users. The sample side can be connected to:
- A second inlet system for variable sample volumes, a multiport with or without a tube cracker installation,
- Microvolumes for very small samples,
- Multisample inlets for separating and purifying samples or,
- Installations for "on-line" coupling to gas chromatographs, elemental analyzer or other peripherals.
Inlet System for MAT 252
The configuration of the inlet system of the MAT 252 mass spectrometer is of asymmetrically design to enable alternately measurements of a sample and a standard gas.
The instrument is equipped with a dual inlet system with a configuration identical for the sample and standard side which enables a balanced intake. Each inlet side has two ports and a variable volume with the respective inlet capillary leading to the changeover valve. For very small samples a microvolume with its own capillary is installed.
Peripherals for MAT 252
ConFlo III Interface
The ConFlo III Interface provides the means for coupling the EA to the IRMS. The connection of the Elemental Analyzer with an isotope ratio mass spectrometer via an open split arrangement has become standard for on-line isotope ratio analysis. The IRMS works with helium flow of ~0.03 ml/min, this gas flow is the central idea of the open split arrangement.
The Open Split Principle
The size of the IRMS capillary determines a 0.3 ml/min gas flow to the mass spectrometer. This MS capillary continuously "sniffs" the carrier gas (He) flowing through the cell. The carrier gas flushes the open split cell continuously with helium and passes the gaseous sample so transporting the produced compounds consecutively through the cell.
Procedure for the ConFlo III Interface
The ConFlo III Interface forms an innovation in coupling an Elemental Analyzer to an IRMS in the way that two separate open split cells replace the "tube in tube" samples gas inlet performed in the predecessor model ConFlo II.
Through the separation of the sample stream and the reference inlet, ConFlo III forms a dynamic and flexible way of an EA/IRMS connection. These two open split cells allow a higher precision of on-line determination as well as a temporal independent sample/reference gas introduction to the IRMS.
CosTech ECS 4010 Elemental Analyzer
The ECS 4010 Nitrogen / Protein Analyzer was designed as an advanced analytical platform for CHNSO elemental analysis and Nitrogen / Protein determination. It is based on an automatic analytical unit whose operation - from sampling up to signal detection - is microprocessor controlled. It represents an evolution in the technique of elemental analysis by "flash combustion/chromatographic separation and multi-detector techniques".
Traditionally, automatic instruments for CHNSO elemental analysis have been divided into two groups; micro and macro elemental analysis. Samples for micro EA are easily analyzed by sophisticated automatic instruments using the technique of combustion/gas chromatography.
For the analysis of stable isotopes of CHNSO, a sampling system is required which does not contaminate the sample with atmospheric nitrogen and oxygen, especially working at very low levels. Advanced, highly stable catalysts are required to avoid any material exchange to the combusted gases.
To increase throughput and minimize maintenance time and cost, a simple approach to the design of the analyzer is essential, especially for macro sample analysis where all combustion gases pass through the detector system without splitting or diluting.
ECS 4010 Description:
Starting from the basic furnace design; the ECS 4010 has two, large capacity (up to a 26 mm OD reactor) furnaces. Optional fittings let you quickly and easily switch between combustion/reduction reactors of different diameters. You can work with reactors from 10,5 mm to 26 mm OD.
The furnaces are mounted in a specially designed SS enclosure with an extremely efficient insulating material. This keeps the entire cabinet "cool to the touch" and allows the furnaces to heat from ambient to operating temperature in 1.5 hours. The electronics and power supplies are completely isolated from the furnace compartment and have their own separate cooling fan. The ECS monitors, and can display, the temperatures of the power supply, and electronics. It will also display the input line voltage supply.
The ECS 4010 consists of three modules:
The first module is the combustion system, the second is the detector system, and the third is the data handling system. When the three systems are combined the user can choose the required analytical configuration.
The first module consists of sampling, combustion and pneumatics. The autosamplers available are a sealed, "Zero Blank" sampler, and an open carousel sampler, where samples can be added continuously. The movement of the "Zero Blank" autosampler is controlled by a microstepping motor. You can choose either a 32 or 50 position carousel through the instrument software, and since it is completely sealed to atmosphere there is no contamination of the samples from nitrogen or oxygen. This is especially useful for stable isotope analysis. The second choice of sampler has carousels which are open to the atmosphere, but samples can be added continuously and the number of sample positions is 147.
The ECS 4010 is equipped with two furnaces. Temperature control of the combustion furnace is up to 1100 °C, and the reduction furnace 800 °C. The furnace dimensions and special fittings allows you to use a wide variety of reactors to tailor the analyzer to your laboratory needs (small and large samples, CHNSO analysis, and N/Protein). The connection fittings for the quartz reactors make it fast and easy to replace the inserts in the combustion reactors (removal of ash and residue), for longer tube and catalyst life. The unique pneumatic system can be switched between three operating modes; Micro, Semi-Micro and Macro. By selecting a mode you choose the volume of oxygen for sample combustion. The gases (helium, oxygen and servo) are controlled by reliable SS diaphragm regulators for accurate flow control. Servo gas control is provided for use with our pneumatically actuated autosampler. A separate purge regulator is provided for flexibility in purge gas flow and volume.
The second module is the detector system for measuring the combustion (or pyrolysis) products. These combustion gases can be separated by gas chromatography for CHNSO analysis, or sent to a mass spectrometer for isotopic analysis. For the gas chromatographic technique a high stability oven, with temperature control from 30-110 °C, and a wide range of GC column lengths are available. The TCD (thermal conductivity detector) has been specially designed for this application, special features include; preheating of combustion gases before entering the detector to stabilize gas temperature, and a twin encapsulated filament which requires no reference gas flow.The advantages of the this are; simplicity of the combustion/detector system (low maintenance, minimum down time), efficiency of the combustion, and no splitting or dilution of the combustion gases, all combustion products are measured.
The third module is EAS32, our WindowsTM based software package, for instrument control, data acquisition and report generation. A multichannel 32 bit A/D interface connects the ECS to a PC for data acquisition. Sample information is entered, the software collects raw data from the TCD, creates the calibration, and generates reports. Data can be easily exported to EXCELTM in ASCII format for postrun calculations or exporting to LIMS. A reprocessing software option is available for setting up methods and transferring data from a remote computer, or working with data out of the laboratory. Interface boards can be ordered for 1, 2 or 4 channels.